Lucia Banci is Professor of Chemistry at the University of Florence. She has an extensive expertise and has provided original contributions and breakthroughs in Structural Biology and in biological NMR. She has addressed and unraveled many aspects of the biology of metal ions in biological systems.
The innovative in cell NMR approach developed by Lucia Banci and her group allows for the detection of human individual proteins in living human cells with atomic level resolution. She also exploited the extensive knowledge of structural biology approaches through NMR expertise to develop an absolutely innovative approach to vaccine design, based on the knowledge of the structure of the pathogen antigens and of the interaction pattern with antibodies, to design structure-based vaccines.
Lucia Banci is one of the founders and present Director of the Center of Magnetic Resonance (CERM) of the University of Florence. She is the Head of the Italian Core Center of the ESFRI Research Infrastructure Instruct-ERIC, and a member of the Instruct-ERIC Executive Committee and of the Council.
Full CV Ita
In-cell NMR, Structural Biology, Structural Vaccinology, Metals in biology, Iron-sulfur protein biogenesis
SELECTED PUBLICATIONS (2010-2022):
Luchinat E, Cremonini M, Banci L. Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR, Chemical Reviews, DOI: 10.1021/acs.chemrev. 1c00790. 2022
Matteucci S, Camponeschi F, Clémancey M, Ciofi-Baffoni S, Blondin G, Banci L. In-cellulo Mӧssbauer and EPR studies bring new evidences to the long-standing debate on the iron-sulfur cluster binding in human anamorsin. Angew Chem Int Ed 60: 14841–14845, 2021
Camponeschi F, Prusty NR, Heider SAE, Ciofi-Baffoni S, Banci L. GLRX3 Acts as a [2Fe-2S] Cluster Chaperone in the Cytosolic Iron-Sulfur Assembly Machinery Transferring [2Fe-2S] Clusters to NUBP1. J Am Chem Soc 142: 10794-10805, 2020
Luchinat E., Barbieri L., Cremonini M., Nocentini A., Supuran C.T., Banci L. Drug screening in human cells by NMR allows early assessment of drug potency, Chem. Int. Ed. 59: 6535 –6539, 2020
Luchinat E, Banci L. In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation. Acc Chem Res 51, 1550-1557, 2018
Camponeschi, F., Ciofi-Baffoni, S., Banci, L. Anamorsin/Ndor1 Complex Reduces [2Fe-2S]-MitoNEET via a Transient Protein-Protein Interaction, J Am Chem Soc, 139: 9479–9482, 2017
Barbieri L, Luchinat E and Banci L. In-cell NMR spectroscopy in HEK293T cells: a protocol to characterize proteins in their physiological environment. Nature Protocols 11: 1101-1111,
Banci L, Ciofi-Baffoni S, Gajda K, Muzzioli R, Peruzzini R and Winkelmann J. N-terminal domains mediate [2Fe-2S] cluster transfer from glutaredoxin-3 to anamorsin. Nat Chem Biol 11: 772-778, 2015.
Banci L, Camponeschi F, Ciofi-Baffoni S and Muzzioli R. Elucidating the molecular function of human BOLA2 in GRX3-Dependent anamorsin maturation pathway. J Am Chem Soc 137: 16133-16134, 2015.
Banci L, Brancaccio D, Ciofi-Baffoni S, Del Conte R, Gadepalli R, Mikolajczyk M, Neri S, Piccioli M and Winkelmann J. [2Fe-2S] cluster transfer in iron-sulfur protein biogenesis, Natl.Acad.Sci.U.S.A, 111, 6203-6208, 2014.
Luchinat E, Barbieri L, Rubino J.T, Kozyreva T, Cantini F & Banci L. In-cell NMR reveals potential precursor of toxic species from SOD1 fALS mutants. Nature Commun. 5, 5502,
Banci L, Barbieri L, Bertini I, Luchinat E, Secci E, Zhao Y & Aricescu A.R. Atomic-resolution monitoring of protein maturation in live human cells by NMR. Nature Chem. Biol. 9, 297-299,
Scarselli M, Aricò B*, Brunelli B, Savino S, Di Marcello F, Palumbo E, Veggi D, Ciucchi L, Cartocci E, Bottomley M.J, Malito E, Lo Surdo P, Comanducci M, Giuliani M.M, Cantini F, Dragonetti S, Colaprico A, Doro F, Giannetti P, Pallaoro M, Brogioni B, Tontini M, Hilleringmann M, Nardi-Dei V, Banci L, Pizza M. & Rappuoli R. Rational design of a meningococcal antigen inducing broad protective immunity. Science Transl Med. 3, 91ra62,
Banci L, Bertini I, Ciofi-Baffoni S, Kozyreva T, Zovo K & Palumaa P. Affinity gradients drive copper to cellular destinations. Nature 465, 645-648,