JACS 2016

A joint structural refinement against NMR data and X-ray diffraction patterns offers unique opportunities to detect differences in the conformation of biomolecules between the solution and the crystal states. Read More

ChemComm

An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1.  Read More

NCB N terminal

Structural and biophysical evidences define the early steps of iron-sulfur protein maturation in the cytosol, demonstrating that  glutaredoxin-3 passes [2Fe-2S] clusters to anamorsin during a protein-protein interaction mediated by their N-terminal domains.  Read More 

nature chemistry

Dynamic nuclear polarization (DNP) in liquid solutions can enhance 13C NMR signals at magnetic fields of 3 T and room temperature up to three orders of magnitude. Read More  Highlighted in Angewandte Chemie

CERM research on covers

cover ACSnano lr

cover J Phys Chem lr  
High relaxivity Gd(III)-DNA gold nanostars: investigation of shape effects on proton relaxation, ACS nano, 9, 3385–3396, 2015 Exploring regions of conformational space occupied by two-domain proteins, J.Phys.Chem.B, 118, 10576-10587, 2014  
Selected_2_august2011

 

Structure-based design of a meningococcal antigen inducing broad protective immunity

Scarselli, M., Arico, B., Brunelli, B., Savino, S., Di Marcello, F., Palumbo, E., Veggi, D., Ciucchi, L., Cartocci, E., Comanducci, M., Giuliani, M. M., Cantini, F., Dragonetti, S., Colaprico, A., Doro, F., Giannetti, P., Pallaoro, M., Brogioni, B., Tontini, M., Hilleringmann, M., Nardi Dei, V., Banci, L.

Sci.Trans.Medicine, 3, 91ra62, 2011

Abstract

The sequence variability of protective antigens is a major challenge to the development of vaccines. For Neisseria meningitidis, the bacterial pathogen that causes meningitis, the amino acid sequence of the protective antigen factor H binding protein (fHBP) has more than 300 variations. These sequence differences can be classified into three distinct groups of antigenic variants that do not induce cross-protective immunity. Our goal was to generate a single antigen that would induce immunity against all known sequence variants of N. meningitidis. To achieve this, we rationally designed, expressed, and purified 54 different mutants of fHBP and tested them in mice for the induction of protective immunity. We identified and determined the crystal structure of a lead chimeric antigen that was able to induce high levels of cross-protective antibodies in mice against all variant strains tested. The new fHBP antigen had a conserved backbone that carried an engineered surface containing specificities for all three variant groups. We demonstrate that the structure-based design of multiple immunodominant antigenic surfaces on a single protein scaffold is possible and represents an effective way to create broadly protective vaccines.

Link to journal online abstract

Link to full article

The article has been evaluated by Carlos Morel new 13 Sep 2011 with Rating 10 - Exceptional

Italian press review (pdf)

International Press review 

http://www.nature.com/nature/journal/v475/n7356/full/475269a.html

http://stm.sciencemag.org/content/3/91/91ps29.full

http://www.fiercevaccines.com/story/novartis-pursues-universal-meningitis-b-vaccine-hemispherix-gets-adjuvant-p/2011-07-21

http://health.usnews.com/health-news/family-health/brain-and-behavior/articles/2011/07/15/researchers-closer-to-developing-universal-meningitis-b-vaccine

http://www.ghtcoalition.org/recent-news-111907-scientists-closer-to-developing-new-meningitis-vaccine.php

http://www.thenewstribe.com/2011/07/17/researchers-closer-to-developing-universal-meningitis-b-vaccine/

http://vaccineinjuryhelpcenter.wordpress.com/2011/07/21/meningitis-b-vaccine-one-step-closer-to-production/

http://nanobiotechnews.com/a-chimeric-protein-mutants-against-pathogens.html

http://www.yalemedicalgroup.org/stw/Page.asp?PageID=STW038742

 http://news.healingwell.com/index.php?p=news1&id=654861 

http://www.easyquicksearch.info/meningitis-b-vaccine-researchers-toward-the-development-of-universal.html 

http://www.goodhealth.com/health_a_to_z/health_news/health_news/2011/07/15/researchers_closer_to_developing_universal_meningitis_b_vaccine

http://chinese.eurekalert.org/en/pub_releases/2011-07/aaft-nvo071111.php http://www.drugs.com/answers/support-group/meningococcal-meningitis-prophylaxis/blog/ 

http://tokillcancer.com/view/Researchers-Closer-to-Developing-Universal-Meningitis-B-Vaccine_15590.html 

http://www.myoptumhealth.com/portal/NewsArchive/item/Researchers+Closer+to+Developing+Univer

https://www.mylifestages.org/healthcenter/News.page?R=hd654861&tid=632+683