The laboratory is very active in the proposal of methodological advancements for high-resolution NMR. Recently, attention has been concentrated on 13C direct-detection applied to the study of macromolecules of biological interest. With an increase in the complexity of the analyzed macromolecules, direct acquisition of the spectra of 13C allows us to push the boundaries of research possible with 1H NMR spectroscopy only.
In this field CERM has contributed to the fine-tuning of a series of 13C-13C and 13C-15N experiments in 2D and 3D at high resolution for the sequence-specific assignment of proteins that are particularly difficult to study using conventional methods.
In the case of proteins containing a paramagnetic metal ion, pseudocontact shifts and residual dipolar couplings are used to refine protein structures and to investigate the extent of the conformational heterogeneity of the system through the maximum occurrence approach.
The results that have been obtained are due in part to an intense collaboration with Bruker, a major company producing NMR instrumentation, which has allowed us to test accessories at the forefront of technology.
Another recent ambition of research at CERM is the study of metalloproteins in the solid phase (solid-state NMR or SS NMR), made possible through the acquisition of an NMR instrument for solid state experiments at 700 MHz (wide-bore magnet). We are finding that large proteins and paramagnetic proteins are potentially better studied in the solid phase than in solution.
Recent studies carried out at CERM have demonstrated that in the solid phase it is possible to observe a paramagnetic shift in metalloproteins analogous to that which is observed in solution, and that this can be used to study structures in the solid phase. These studies have been carried out on a wide-bore 700 MHz instrument using an MAS probe in triple resonance 4mm capable of keeping the specimen in rotation up to 15 kHz. An MAS triple resonance 3.2 mm probe that permits operation at a higher speed (up to 25 kHz) has recently been acquired.

Additionally, low field NMR (relaxometry) has been in continuous development to facilitate the understanding of the fine details of electron-nucleus coupling.