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Bioinformatics & Computational Structural Biology At CERM the research in the field of Bioinformatics includes: Construction of a database of metalloproteins (metallome); Analysis of weak interactions among proteins; Comparative analyses of genomic sequences and genetic data banks; Evolutionary analyses of metalloprotein families; Simulations of molecular dynamics of metalloproteins In silico docking of candidate pharmaceuticals against selected pharmacological targets. Read More
Drug Design, Chemical Synthesis & Library Screening One of our top areas of research focuses on the design and development of new pharmaceuticals and the identification of new therapeutic targets. Read More
Fast Field Cycling Relaxometry Using a fast field cycling relaxometer, we measure the relaxation profiles of solvent water protons in the presence of paramagnetic complexes, e.g. nitroxides, or diamagnetic or paramagnetic proteins. Read More
Metabolomics by NMR and MS Metabolomics is the study of the metabolome or a set of metabolites (i.e. small molecules) produced by an organism in biological fluids such as serum, plasma and urine. The projects involve varied areas of interest, in particular the biomedical field. Read More
NMR Developments The development of new NMR methods is essential to broadening the range of NMR spectroscopy applications in the study of different systems. Read More
Paramagnetism-assisted NMR
The presence of a paramagnetic ion within a biomolecule (either naturally present or introduced by metal substitution in diamagnetic metalloproteins or by attachment of a paramagnetic tag) induces pseudocontact shifts, residual dipolar couplings and/or paramagnetic relaxation enhancements. Read More
Protein Expression & Isotope Labelling Both fusion and non fusion proteins are usually prepared. Protein purification can easily be accelerated by using FPLC.Read More
Standard Operating Procedures for Cryopreservation & Biobanking (via NMR) Biosamples should be collected and stored according to standardized protocols (Standard Operating Procedures - SOPs) guaranteeing uniformity of all samples of a specific type (concerning quality and biological integrity), in order to represent a good starting material for scientific experiments, as similar as possible to freshly collected samples. Read More