luchinatphoto 

 

Dr. Claudio Luchinat
Professor of Chemistry
Magnetic Resonance Center
and Department of Chemistry
University of Florence
Via L. Sacconi 6
50019 Sesto Fiorentino, (FI) - Italy

Tel: +39 055 4574296 - Fax: +39 055 4574923

E-mail: luchinattomtecerm.unifi.it

 

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Curriculum Vitae (English)

 

List of Publications

 

Educational

 

Full Professor of Chemistry at the University of Florence, co-founder and present Director of the Center of Magnetic Resonance (CERM), co-founder and present Director and President of the Interuniversity Consortium on Magnetic Resonance of Metallo Proteins (CIRMMP).


His research interests include bioinorganic chemistry, structural biology, development of NMR-based structural methodologies in solution and in the solid state, metalloproteins and metalloenzymes, spectroscopy, theory of electron and nuclear relaxation, NMR of paramagnetic species, relaxometry, contrast agents and NMR-based analytical methods in general. Particularly worth mentioning are his recent studies on the integration of structural techniques, where he has shown that simultaneous refinement of X-ray and NMR data can lead to i) a significant increase of accuracy of the resulting structural data and ii) a robust strategy to assess if structural differences exist for a biomolecule between solution and crystalline state. He has also significantly contributed to the theoretical understanding of the phenomenon of Dynamic Nuclear Polarization (DNP), both in solids and in liquids. DNP is a promising strategy to enhance the sensitivity of the NMR experiment, possibly transforming NMR into a completely new technique for the investigation of biological systems in ways that would have been unconceivable only a few years ago.
Starting from 2008, his research has been also directed towards metabolomics, aiming at obtaining the metabolic profiles of biological fluids such as urines and blood using NMR spectroscopy; defining procedures for sample preparation and for the acquisition of NMR spectra; developing statistical methods for the analysis of the data; and correlating the metabolic profiles with pathophysiological characteristics of the subjects studied. The results of this research, published in international journals, have been cited several times not only in specialized literature but also in other sectors, in books, websites and also in non-specialist press. Claudio Luchinat has become one of the international reference points for metabolomics, and is invited to give plenary lectures on the topic at conferences both specialized and in other sectors. He is invited as a keynote speaker from research laboratories in various countries around the world, and invited to inaugurate new research centers and research infrastructures.


He is the author of over 600 publications, written in English, in scientific journals of international renown. Together with S. Aime and G. Valensin he is the author of the book (in Italian): “NMR Spectroscopy: Applications in Inorganic and Bioinorganic Chemistry, Italian Association of Inorganic Chemistry”, 1984. Together with I. Bertini he is the author of the book: “NMR of Paramagnetic Molecules in Biological Systems”, Benjamin-Cummings, 1986. Together with I. Bertini and L. Banci he is the author of the book: “Nuclear and Electron Relaxation”, VCH, 1991. Together with I. Bertini he is the author of the book: “NMR of Paramagnetic Substances”, Elsevier, 1996. Together with I. Bertini and G. Parigi he is the author of the book “Solution NMR of Paramagnetic Molecules”, Elsevier, 2001. Together with I. Bertini, G. Parigi and E. Ravera he is the author of the book “NMR of Paramagnetic Molecules”, Elsevier, 2017.

Selected recent publications

Liu, G., Levien, M., Karschin, N., Parigi, G., Luchinat, C., and Bennati, M., One-thousand fold enhancement of high field liquid nuclear-magnetic resonance signals at room temperature, Nat.Chem., 9, 676-680, 2017.

 

Takis, PG; Schaefer,H.; Spraul, M.; Luchinat,C.; Deconvoluting interrelationships between concentrations and chemical shifts in urine provides a powerful analysis tool Nat.Comm., doi:10.1038/ s41467-017-01587-0, 1662, 2017.

 

Giuntini, S., Balducci, E., Cerofolini, L., Ravera, E., Fragai, M., and Berti, F., Luchinat,C., Characterization of conjugation pattern in large polysaccaride-protein conjugates by NMR, Angewandte Chemie, 56, 14997-15001, 2017.

 

Lal, P., Cerofolini, L., D'Agostino, VG, Zucal, C., Fuccio, C., Bonomo, I, Dassi, E., Giuntini, S., Di Maio, D., Vishwakarma, V., Preet, R., Williams, SN, Fairlamb, MS, Munk, R, Lehrmann, E, Abdelmohsen, K, Elezgarai, SR, Luchinat, C., Novellino, E., Quattrone, A., Biasini, E, Manzoni, L, Gorospe, M, Dixon, DA, Seneci, P, Marinelli, L., Fragai, M., and Provenzani, A., Regulation of HuR structure and function by dihydrotanshione-I, Nucleic Acids Res., 45, 9514–9527, 2017.

 

Andralojc, W., Hiruma, Y., Liu, W.-M., Ravera, E., Nojiri, M., Parigi, G., Luchinat, C., and Ubbink, M., Identification of productive and futile encounters in an electron transfer protein complex, Proc.Natl.Acad.Sci.USA, 114, E1840-E1847, 2017.

 

Rammohan, N., MacRenaris, K. W., Moore, L., Parigi, G., Mastarone, D. J., Manus, L. M., Lilley, L., Preslar, A., Waters, E. A., Filicko, A., Luchinat, C., Ho, D. and Meade, T.J., Nanodimond-gadolinium(III) aggregates for tracking cancer growth in vivo at high filed, Nano Letters, 16: 7551–7564, 2016.

 

Carlon, A., Ravera, E., Hennig, J., Parigi, G., Sattler, M. and Luchinat, C., Improved accuracy from joint X-ray and NMR refinement of a protein-RNA complex structure, J.Am.Chem.Soc., 138: 1601-1610, 2016.

 

Ravera, E., Ciambellotti, S., Cerofolini, L., Martelli, T., Kozyreva, T., Bernacchioni, C., Giuntini, S., Fragai, M., Turano, P. and Luchinat, C., Solid-state NMR of PEGylated proteins, Angew.Chem.Int.Ed., 55: 1-5, 2016.

 

Benda, L., Mareš, J., Ravera, E., Parigi, G., Luchinat, C., Kaupp, M., and Vaara, J., Pseudo-contact NMR shifts over a paramagnetic metalloprotein (CoMMP-12) from first principles, Angew.Chem.Int.Ed., 55, 14713-14717, 2016.

 

Rotz, M. W., Culver, K. S. B., Parigi, G., MacRenaris, K. W., Luchinat, C., Odom, T. W., and Meade, T. J., High relaxivity Gd(III)-DNA gold nanostars: invstigation of shape effects on proton relaxation, ACS nano, 9, 3385-3396, 2015.

 

Ardenkjaer-Larsen, J.-H., Boebinger, G. S., Comment, A., Duckett, S. B., Edison, A. S., Engelke, F., Griesinger, C., Griffin, R. G., Hilty, C., Maeda, H., Parigi, G., Prisner, T. F., Ravera, E., van Bentum, G. J. M., Vega, S., Webb, A., Luchinat, C., Schwalbe, H. and Frydman, L. Facing and overcoming biomolecular NMR's sensitivity challanges, Angew.Chem.Int.Ed., 54, 9162-9185, 2015.

 

Ravera, E., Salmon, L., Fragai, M., Parigi, G., Al-Hashimi, H. M., and Luchinat, C., Insights into domain-domain motions in proteins and RNA from solution NMR, Acc.Chem.Res., 47, 3118-3126, 2014.

 

Rinaldelli, M., Ravera, E., Calderone, V., Parigi, G., Murshudov, G. N., and Luchinat, C., Simultaneous use of solution NMR and X-ray data REFMAC5 for joint refinement/detection of structural differences, Acta Cryst.D, D70, 958-967, 2014.

 

Luchinat, C., Parigi, G., and Ravera, E., NMR technology: the competitive world of RAS biology, Nature Chem.Biol., 10, 173-174, 2014.

 

Preslar, A t., Parigi, G., McClendon, M. T., Sefick, S. S., Moyer, T. J., Haney, C. R., Waters, E. A., MacRenaris, K. W., Luchinat, C., Stupp, S. I., and Meade, T. J., Gd(III)-labeled peptide nanofibers for reporting on biomaterial localization in vivo, ACS nano, 8, 7325-7332, 2014.

 

Corzilius, B., Michaelis, V. K., Penzel, S., Ravera, E., Smith, A., Luchinat, C., and Griffin, R. G., Dynamic nuclear polarization of 1H, 13C and 59Co in a Co(III) tris-ethylenediamine crystalline lattice doped with Cr(III), J.Am.Chem.Soc., 136, 11716-11727, 2014.

 

Parigi, G., Rezaei-Ghaleh, N., Giachetti, A., Becker, S., Fernandez, C., Blackledge, M., Griesinger, C., Zweckstetter, M., and Luchinat, C., Long-Range Correlated Dynamics in Intrinsically Disordered Proteins, J.Am.Chem.Soc., 136, 16201-16209, 4-11-2014.

 

Tarocchi, M., Polvani, S., Peired, AJ, Marroncini, G., Calamante, M., Ceni, E., Rhodes, D., Mello, G., Pieraccini, G., Quattrone, A., Luchinat, C., and Galli, A., Telomerase activated thymidine analogue pro-drug is a new molecule targeting hepatocellular carcinoma, J.Hepatol., 14, 1064-1072, 2014.

 

Cacciatore, S., Luchinat, C., and Tenori, L., Knowledge discovery by accuracy maximization, Proc.Natl.Acad.Sci.USA, 111, 5117-5122, 2014.

 

Cerofolini, L., Amato, J., Fragai, M., Giachetti, A., Limongelli, V., Luchinat, C., Novellino, E., Parrinello, M., and Randazzo, A., G-triplex structure and formation propensity, Nucleic Acids Res., 42, 13393-13404, 2014.

 

Bertini, I., Luchinat, C., Parigi, G., and Ravera, E., SedNMR: on the edge between solution and solid state NMR, Acc.Chem.Res., 46, 2059-2069, 2013.

 

Limongelli, V., De Tito, S., Cerofolini, L., Fragai, M., Pagano, B., Trotta, R., Cosconati, S., Marinelli, L., Novellino, E., Bertini, I., Randazzo, A., Luchinat, C., and Parrinello, M., The G-triplex DNA, Angew.Chem.Int.Ed., 52, 2269-2273, 2013.

 

Fragai, M., Luchinat, C., Parigi, G., and Ravera, E., Conformational freedom of metalloproteins revealed by paramagnetism-assisted NMR, Coord.Chem.Rev., 257, 2652-2667, 2013.

 

Ravera, E., Corzilius, B., Michaelis, V. K., Rosa, C., Griffin, R. G., Luchinat, C., and Bertini, I., Dynamic nuclear polarization of sedimented solutes, J.Am.Chem.Soc., 135, 1641-1644, 2013.

 

Luchinat, C., Parigi, G., Ravera, E., Rinaldelli, M., Solid state NMR cristallography through paramagnetic restraints,  J.Am.Chem.Soc., 134, 5006-5009, 2012.

 

Bertini, I., Fragai, M., Luchinat, C., Melikian, M., Toccafondi, M., Lauer, J.L., Fields, G.B., Structural basis for metalloproteinase 1-catalyzed collagenolysis, J.Am.Chem.Soc., 134, 2100-2112, 2012.

 

Bertini, I., Luchinat, C., and Parigi, G., Ravera, E., Reif, B., Turano, P., Solid-state NMR of proteins sedimented by ultracentrifufation ,  Proc.Natl.Acad.Sci.USA, 108, 10396-10399, 2011.

 

Bertini, I., Gonnelli, L., Luchinat, C., Mao, J., Nesi, A., A new structural model Aß40 fibrils, J.Am.Chem.Soc., 133, 16013-16022, 2011.

 

Bertini, I., Cacciatore, S., Jensen, B.V., Schou, J.V., Johansen J.S., Kruhøffer, M., Luchinat, C., Nielsen, D. L., and Turano, P., Metabolomic NMR fingerprinting to identify and predict survival of patients with metastatic colorectal cancer,  Cancer Res., 356-364, 2012.

 

Bertini, I., Luchinat, C., and Parigi, G., Moving the frontiers in solution solid state bioNMR. A celebration of Harry Gray's 75th birthday,  Coord.Chem.Rev., 255, 649-663, 2011.


Banci, L., Bertini, I., Blazevits, O., Cantini, F., Lelli, M., Luchinat, C., Mao, J., and Vieru, M., NMR characterization of a "fibril-ready" state of demetallated wild-type superoxide dismutase,  J.Am.Chem.Soc., 133, 345-349, 2011.


Mastarone, D. J., Harrison, V. S. R., Eckermann, A. L., Parigi, G., Luchinat, C., and Meade, T. J., A modular system for the synthesis of multiplexed MR probes,  J.Am.Chem.Soc., 133,5329-5337, 2011.

Corzilius, B., Smith, A.A., Barnes, A.B., Luchinat, C., Bertini, I., Griffin, R.G., High-Field Dynamic Nuclear Polarization with High-Spin Transition Metal Ions, J. Am. Chem. Soc., 133, 5648-5651, 2011.

Bertini, I., Luchinat, C., Spectroscopy: Unexpected interactions, Nature, 470:469-470, 2011.

 

Polvani, S., Calamante, M., Foresta, V., Ceni, E., Mordini, A., Quattrone, A., D'Amico, M., Luchinat, C., Bertini, I., and Galli, A., Acycloguanosyl 5'-thymidyltriphosphate, a new thymidine analogue pro-drug for pancreatic cancer therapy, Gastroenterology140, 709-720, 2011.

 

Bertini, I., Giachetti, A., Luchinat, C., Parigi, G., Petoukhov, M. V., Pierattelli, R., Ravera, E., and Svergun, D. I., Conformational space of flexible biological macromolecules from average data, J.Am.Chem.Soc., 132, 13553-13558, 2010.

 

Bertini, I., Emsley, L., Lelli, M., Luchinat, C., Mao, J., and Pintacuda, G., Ultra-Fast MAS solid-state NMR permits extensive 13C and 1H detection in paramagnetic metalloproteins, J.Am.Chem.Soc., 132, 5558-5559, 2010.

 

Bertini, I., Bhaumik, A., De Paepe,G., Griffin, R. G., Lelli, M., Lewandowski, J., and Luchinat, C., High-resolution solid state NMR structure of a 17.6 kDa protein, J. Am. Chem. Soc., 132: 1032-1040, 2010.

 

Bertini, I., Kursula, P., Luchinat, C., Parigi, G., Vahokoski, J., Willmans, M., and Yuan, J., Accurate solution structures of proteins from X-ray data and minimal set of NMR data: calmodulin peptide complexes as examples, J.Am.ChemSoc., 131: 5134-5144, 2009

 

Reese, M., Tuerke, M.-T, Tkach, I., Parigi, G., Luchinat, C., Marquardsen, T., Tavernier,A., Hofer, P., Engelke, F., Griesinger, C., and Bennati, M., 1H and
13C dynamic nuclear polarization in aqueous solution with a two-field (0.35T/14T) shuttle DNP spectometer, J.Am.Chem.Soc., 131: 15086-15087, 2009.

 

Pellecchia, M., Bertini, I., Cowburn, D., Dalvit, C., Giralt, E., Jahnke, W., James, T. L.,Homans, S. W., Kessler, H., Luchinat, C., Meyer, B., Oschkinat, H., Peng, J., Schwalbe, H.,and Siegal, G., Perspectives on NMR in drug discovery: a technique comes of age, Nature Rev.Drug Discov., 7: 738-745, 2008.

 

Bertini, I., Fragai, M., Luchinat, C., and Talluri, E., Water-based ligand screening for paramagnetic metalloproteins, Angew.Chem.Int.Ed., 47: 4533-4537, 2008

 

Balayssac, S., Bertini, I., Bhaumik, A., Lelli, M., and Luchinat, C., Paramagnetic shifts in solid-state NMR of proteins to elicit structural information., Proc.Natl.Acad.Sci.USA, 105: 17284-17289, 2008.

 

Bertini, I., Calderone, V., Fragai, M., Jaiswal, R., Luchinat, C., Melikian, M., Mylonas, E., and Svergun, D., Evidence of reciprocal reorientaion of the catalytic and hemopexin-like domains of full-length MMP-12, J.Am.Chem.Soc.: 130, 7011-7021, 2008

 

Assfalg, M., Bertini, I., Colangiuli, D., Luchinat, C., Schaefer, H., Schuetz, B., and Spraul, M., Evidence of different metabolic phenotypes in humans, Proc. Natl. Acad. Sci. USA. 105:1420-1424, 2008.

 

Luchinat, C. and Parigi, G., Collective relaxation of protein protons at very low magnetic field: a new window on protein dynamics and aggregation., J.Am.Chem.Soc., 129, 1055-1064, 2007.

 

Bertini, I., Gupta, Y. K., Luchinat, C., Parigi, G., Peana, M., Sgheri, L., and Yuan, J, Paramagnetism-Based NMR Restraints Provide Maximum Allowed Probabilities for the Different Conformations of Partially Independent Protein Domains, J.Am.Chem.Soc., 129, 12786-12794, 2007.

 

Bertini, I., Calderone, V., Fragai, M., Giachetti, A., Loconte, M., Luchinat, C., Maletta, M., Nativi, C., and Yeo, K. J., Exploring the subtleties of drug-receptor interactions: the case of matrix metalloproteinases, J.Am.Chem.Soc., 129, 2466-2475, 2007.

 

Balayssac, S., Bertini, I., Lelli, M., Luchinat, C., and Maletta, M., Paramagnetic ions provide precious structural restraints in solid-state NMR ofproteins, J.Am.Chem.Soc., 129, 2218-2219, 2007.

 

Major, J. L., Meade, T. J., Parigi, G., and Luchinat, C., The Synthesis and In-Vitro testing of a Zn(II)-Activated MR contrast agent, Proc.Natl.Acad.Sci.USA, 104, 13881-13886, 2007.

 

Bermel, W., Bertini, I., Felli, I. C., Lee, Y.-M., Luchinat, C., and Pierattelli, R., Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins, J.Am.Chem.Soc. , 128: 3918-3919, 2006.

 

Balayssac, S., Bertini, I., Luchinat, C., Parigi, G., and Piccioli, M., 13C direct detected NMR increases the detectability of residual dipolar couplings, J.Am.Chem.Soc. , 128: 15042-15043, 2006.

 

Bertini, I., Calderone, V., Fragai, M., Luchinat, C., Maletta, M., and Yeo, K. J., Snapshots of the Reaction Mechanism of Matrix Metalloproteinases, Angew.Chem.Int.Ed , 45, 7952-7955, 2006.

 

Fragai, M., Luchinat, C., and Parigi, G., "Four-dimensional" protein structures: examples from metalloproteins, Acc.Chem.Res. , 39: 909-917, 2006.