In-cell NMR analysis of biomolecular structure and function

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Instruct Training Course
December 2-6, 2013
Magnetic Resonance Center
Florence - Italy

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The aim of the course is to train young researchers in the use of in-cell NMR spectroscopy, an innovative tool having a great potential to reveal biomolecular structure, dynamics and interactions in living cells. Students will acquire the necessary expertise on how to handle living cell NMR samples and understand critical parameters for detecting and studying proteins and nucleic acids. They will have the opportunity to perform the experiments on their own and familiarise with “faster than death” NMR techniques, which enable the recording of complete NMR data sets for the analysis of short-lived samples. Future challenges of chemical and structural biology to be tackled by in-cell NMR will be discussed.

Course organizers: 

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GIDRM

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Sponsors:

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Outline of course content

The course will start at 2.00 pm on Monday 2nd and will close on Friday 6th in the afternoon.

Lectures

  • Protein expression in prokaryotic and eukaryotic systems
  • Tagged protein expression/delivery into mammalian systems
  • The choice of labelling
  • Fast NMR pulse sequences
  • Protein dynamics in living cells (relaxation measurements, deuterium exchange)
  • Assignment strategies and structure calculations using in-cell NMR data
  • Post-translational modifications and interactions (with drugs, metal ions and other proteins)
  • In-cell NMR of nucleic acids

 

Practicals

  • Tips and tricks of cell sample preparation
  • Experimental setup of an in-cell NMR experiment
  • Cell viability and protein leakage assessment
  • In-cell interactions via NMR

 

Click here to download the Course programme

 

Speakers/Tutors

Peter B. Crowley, School of Chemistry, National University of Ireland Galway
Topic: Protein dynamics/interactions in living cells

Volker Dötsch, Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University (Frankfurt/Main)
Topics: In-cell Solid State NMR / Protein expression in prokaryotic systems

Enrico Luchinat, CERM and Department of Chemistry, University of Florence
Topic: Protein expression in mammalian systems

Philipp Selenko, In-Cell NMR Laboratory, Leibniz Institute of Molecular Pharmacology (Berlin),
Topics: Protein delivery into mammalian systems / Post-translational modifications

Lukas Trantirek, Bijvoet Centre for Biomolecular Research, Utrecht University & Central European Institute of Technology, Masaryk University (Brno) 
Topic: In-cell NMR of nucleic acids

 

Registration fee: 300 €

The fee includes the course material, lunches and coffee breaks during the course, plus the social dinner.

Instruct is providing support for attendees from countries that formally joined Instruct.

To apply, please complete the application form and attach your CV ( http://www.cerm.unifi.it/static/incell-registration/registration.html).

Up to twenty places are available.

 

Deadline for application: October 25th, 2013

 

Contacts

Fabio Arnesano: fabio.arnesanotomteuniba.it
Francesca Morelli: fmorellitomtecerm.unifi.it