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The CERM infrastructure, established in Florence in the new scientific Campus of the University, provides a unique environment for research in the field of Bio-NMR, with an emphasis on structural biology. The infrastructure is equipped with: § Highest magnetic field available (900 MHz), with 900 MHz TCI cryo, optimized for 1H and 13C direct detection § Highest fields equipped with cryoprobes (900 MHz TCI cryo, 800 MHz TXI cryo, 700 MHz TCI cryo) § Highest magnetic field available for Solid State (850 MHz), UltraShield UltraStabilized, with 850 MHz 3.2mm DVT three channels, for 13C, 15N and 1H detection. § Widest range of magnetic fields available which allows a user to combine both high resolution NMR (600 MHz TCI HCP cryo, 500 MHz TCI cryo, 400 MHz and 200 MHz) and relaxometry (down to .01 MHz) § BBO probe at 600 MHz for low frequency heteronuclei to meet the most disparate experimental needs § Liquid handler system and autosamplers for metabonomics and ligand screening § Solid state NMR, particularly with microcrystalline samples § Unique expertise in paramagnetic systems, both in solution and solid-state Special accessories such as the high power probe (short 90° pulse) at 600 MHz and prototype probeheads optimized for 13C direct detection at 700 MHz are available. All of the high field NMR instruments are state-of-the-art digital spectrometers of the Bruker Avance series. In addition to high field NMR spectrometers, the Florence infrastructure is equipped with several instruments to perform low-field NMR studies. A low-resolution instrument operating between 0.01 and 40 MHz is available. For specialists, we can summarize machines and accessories: SPECTROMETERS | | PROBES | | ACCESSORIES | 900 MHz | | 5 mm TCI cryo 1H-13C/15N-2H Z-GRD
5 mm TXI cryo 1H-13C/15N-2H Z-GRD | | | | 850 MHz wide bore | | 3.2 mm MAS DVT 15N/13C/1H for fast spinning (MAS = 25 KHz)
1.3 mm MAS 1H-19F/BB/15N | | Special Air Drier Device (X-AD) BCU-Xtreem cooling device, insert line equipped with external Lock System | 800 MHz | | 5 mm TXI cryo 1H-13C/15N-2H Z-GRD,
2.5 mm HP SEL 1H for paramagnetic spectroscopy
5 mm TXI 1H-2H/13C-15N Z-GRD,
5 mm QXI 1H-31P/13C/15N XYZ-GRD | | | 700 MHz wide bore | | 3.2 mm MAS 1H,15N,13C
4 mm MAS 1H,15N,13C | | | 700b MHz | | 5 mm TXO probe for direct 13C detection 13C/15N-1H-2H Z-GRD
5 mm TXO cryo probe for direct 13C detection 13C/15N-1H-2H Z-GRD | | | 700 MHz | | 5 mm TCI cryo 1H-13C/15N-2H Z_GRD
5 mm TXI 1H-2H/13C-15N Z-GRD,
5 mm TXI 1H-2H/13C-15N XYZ-GRD | | Standard BACS120 autosampler | 600 MHz | | 5 mm TXI 1H-2H/13C-15N Z-GRD,
4 mm HR-MAS 1H-13C/15N/2H MAS-GRD
5 mm SEL 1H,
5 mm SEL 1H/HP,
5 mm BBO,
10 mm BBO,
10 mm BB (LowFreq)
5 mm BBI | | Standard BACS120 autosampler and a Bruker liquid handler system
40 Grad. Ampl. for diffusion measurements | 600b MHz | | 5 mm TXI 1H-2H/13C-15N Z-GRD with ATMM
5 mm TCI cryo 1H-31P/13C/D Z GRD with ATMM | | | 500 MHz | | 5 mm TCI cryo 1H-13C/15N-2H Z-GRD
5 mm TXI 1H-2H/13C-15N Z-GRD
5 mm BBI | | | 400 MHz | | 5 mm BBO Z-GRD,
5 mm SEL 1H for paramagnetic spectroscopy | | |
RELAXOMETERS | FIELD | FFC Stelar | 10 kHz - 40 MHz | 90 MHz Bruker | 4 - 90 MHz |
The computing power (PC clusters and a large number of networked UNIX and Linux workstations) is continuously upgraded to satisfy the needs of scientists working on data processing, structure determination, data analysis, molecular modeling, docking, and MD simulations. Bioinformatics tools are available and are under development. Thanks to widespread international activities, a virtual lab is available to the users of CERM and EU-NMR to perform calculations on the NMR-Life cluster of PCs. The CERM Infrastructure offers access to companies and Public Research Institutions. This service is operated by local technical staff, and special agreements can be reached with partners. The Consorzio Internuniversitario Risonanze Magnetiche di Metallo Proteine Paramagnetiche (CIRMMP) provides some free access to Public Institutions and, for preliminary measurements, to companies. To access the Infrastructure, please complete the form, linked to below. Interactive form for access to instrumentation Coordination Action NMR-Life
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